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Fig. 2 Expression of smad5 and smad1. (A) Developmental northern analysis with 10 μg of total RNA of the indicated stages, hybridized with a smad5- (upper panel) and a smad1- (middle panel) specific probe. Lower panel shows ethidium bromide staining of 28S rRNA as loading control. (B-H) Whole-mount in situ hybridization using a smad5-specific probe. (B) 4-cell stage, animal view. (C) Sphere stage, lateral view. (D) Shield stage, lateral view, dorsal right. (F) 80% epiboly, lateral view, dorsal right.
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