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Fig. 3 ace(fgf8) expression is upregulated in aus mutant embryos. Lateral views of whole embryos. (A,B) Sphere stage. ace expression in the blastula margin is upregulated in the putative aus mutant embryo (B) as compared to a putative wild-type sibling (A). (C-F) ace expression is upregulated at many sites of expression in 18-somite stage (C,D) and prim-12 stage (E,F) aus mutant embryos (D,F) as compared to wild-type siblings (C,E). Inset panels in C,D are close-up pictures of ace expression in the somites and tailbud. (G,H) By the fourth day of development, there is no apparent difference in the expression levels of ace in the aus mutant embryo (H) as compared to a wild-type sibling (G). Abbreviations: dd, dorsal diencephalon; m, margin; mhb, midbrain/hindbrain boundary; os, optic stalks; s, somites; t, telencephalon; tb, tailbud; wt, wild-type. Scale bars: (A-D) 125 μm; (E-H) 100 μm.