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Fig. S1
Characterisation of MTN neuronal development. DiI labelling (A,D) of jaw muscles and GFP (B,E) in Tg[dlx5a/6a:egfp] 5 dpf larvae reveals that a proportion of MTN neurons (arrowheads) co-express GFP and are larger than adjacent tectal GFP+ neurons (arrow, C,F). At 24 hpf many huc /elavl3 expressing progenitor cells are present along the A-P extent of the midbrain (G). In contrast drg11 expressing MTN (arrowheads) are restricted to the pax7 expressing midbrain (I) in comparison to nTPC neurons present in the pax6+ forebrain (H). In Tg[elavl3:gfp] embryos at 24 hpf MTN and nTPC neurons are GFP+ and express drg11 (J), Isl1 (K) and HuC protein (L). Scale bars 100 μm (A-C,G-K) 50μm (L) 20μm (D-F).