ZFIN Image: Bräutigam et al., 2013, Fig. S1

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Figure Caption

Fig. S1

Knockdown of zfGrx2 does not induce gross changes in morphology of zebrafish embryos. (A) At 48 hpf, fli:EGFP transgenic embryos displayed malformation (arrows) of the fluorescent vasculature after morpholino induced knockdown of zfGrx2. The overall morphology was unaffected by the loss of zfGrx2. (Scale bar, 200 μm.) (B) Densitometric analyses of three Western blots revealed significant down-regulation of zfGrx2 at 48 hpf after morpholino injection. (C) Visualization of cell death by Acridine Orange staining in fli:EGFP control embryos and zfGrx2 knockdown embryos with confirmed defects in vessel formation 48 hpf. (Scale bars, 100 μm.)

Acknowledgments

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