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Fig. 1 Teneurin-3 Is Expressed in Interconnected Regions of the Zebrafish Visual System
(A–C) Retinal cryosections of whole-mount in situ hybridizations showing tenm3 mRNA expression at 2, 3, and 5 dpf.
(D and H) Control in situ hybridizations using sense tenm3 riboprobe.
(E–G) Tectal cryosections of whole-mount in situ hybridizations showing tenm3 mRNA expression at 2, 3, and 5 dpf. All images are in transverse plane. Scale bar, 40 μm. N, neuropil; M, medial; V, ventral.
(I) Schematic showing the expression pattern of tenm3 in the retina. Tenm3-positive cells are represented as blue circles. Neuropil layers are indicated in gray. Anatomical reference is reported on the right. IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer.
(J) Schematic showing the expression pattern of tenm3 in the optic tectum.
(K) Schematic detailing the targeting site of splice-blocking tenm3 morpholino (MO), which is shown in red. Exons are represented in cyan. Solid lines indicate introns. The dashed line indicates exon 3 deletion caused by tenm3 MO injections. Primers used for RT-PCR (L) are reported as blue arrows.
(L) RT-PCR analysis of tenm3 mRNA structure in control MO- and tenm3 MO-injected embryos. Two shorter splice variants are distinguished in tenm3 morphants. cDNA sequence comparison revealed that the shortest splice variant lacks exon 3.
(M) Schematic detailing the effect of exon 3 deletion caused by the splice-blocking tenm3 MO, resulting in the deletion of Tenm3 transmembrane and extracellular domains. The full-length protein is represented on the left. The N terminus is located intracellularly, whereas the C terminus is in the extracellular space.
(N–P) At 4 dpf, tenm3 morphant larvae fail to visually adapt their skin pigmentation to the level of background illumination.
See also Figure S1.