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Fig. 4 cvl2 is required for NC induction. (A) ISH on Tg(BRE:mRFP) embryos injected with 8 ng control MO or either 4 ng or 8 ng of cvl2 MO1. Dorsal anterior views, with anterior to the top. Embryos were fixed at the 4-somite stage. (B) Western blot analysis showing dose-dependent reduction of PSmad1/5 levels in the cvl2 morphants at 80% epiboly. (C) ISH on Tg(hsp70l:dnXla.Bmpr1a-GFP) embryos heat shocked at bud stage using probes directed against sox10 and cvl2.
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