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Fig. S4
Anterior transcription factor overexpression has little or no effect on pectoral fin regeneration. (A) Expression of alx4a, id4 or lhx9 is induced in posterior pectoral fin regions at 10-fold or higher levels in new transgenic lines, 4 hours after a single heat-shock at 4 dpa. Values are normalized to actb1 levels and relative to wild-type controls. n=3; **P<0.005, Student’s t-test; mean ± s.e.m. (B) Appearance of transgenic and wild-type clutchmate fins at 7 dpa after a series of daily heat shocks. Transgenic fins appear similar to wild-type fins. (C) Quantification of regenerate lengths indicates a minor change in some rays after anterior gene overexpression. n=12-16; *P<0.05, Student’s t-test. (D) Quantification of width ratios indicates a minor change in some rays. n=12-16; *P<0.05, Student’s t-test. Scale bars: 0.5 mm.