ZFIN Image: Rosenfeld et al., 2013, Fig. S4

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Figure Caption

Fig. S4

Validation of the splice-blocking morpholinos. MOs targeting hspb7 or hspb12 transcripts demonstrate specific knockdown of each gene product. A) Schematic demonstrating the target splice sites for each morpholino. The 7MO targets the splice acceptor site of exon 1 of the hspb7 transcript while 12MO targets the splice donor site of exon 2 of the hspb12 transcript. Primers used to generate amplicons are also indicated. B) Semi-quantitative PCR of the amplicons generated by primer pairs for hspb7 (TE4546 and TE4547) and hspb12 (TE4548 and TE4549). Transcripts are largely depleted when MOs were used at 0.3mM and above, which correlates to the concentrations used to generate phenotypes in our experiments.

Acknowledgments

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Reprinted from Developmental Biology, 381(2), Rosenfeld, G.E., Mercer, E.J., Mason, C.E., and Evans, T., Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis, 389-400, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.