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Fig. S7
The cardiomyopathy caused by depletion of Tmem88a is p53 independent. Brightfield images of representative 48-hpf p53–/– embryos injected with (A) control (Ctrl MO) or (B) tmem88a (T88a MO) morpholino. Images on the right (insets) are higher magnifications of cardiac and cranial tissues in each embryo. All views are lateral, anterior to the left. In the main panels, blood flow is obvious in the p53 mutant (arrow in A), but absent in the tmem88a morphant, with pericardial edema (arrowhead in B). Brain (br), heart (hrt) and blood (bl) are indicated and a translucent heart-string is evident in the tmem88a morphant. n>50 embryos analyzed per panel. Scale bar: 0.1 mm in insets.