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Fig. S1
Analysis of tmem88a expression in embryos lacking hematovascular progenitors. WISH for tmem88a and the notochord marker ntl in sibling (A) wild-type (WT) (n=17) and (B) cloche (n=6) embryos at the 10-somite stage. Shown are dorsal views of flatmounted embryos, with anterior to the left. Homozygous cloche mutants lack tmem88a expression in the hematovascular region of the posterior lateral plate mesoderm (arrowheads). By contrast, as confirmed in higher magnification views of boxed areas, tmem88a transcript patterns are normal in the ALPM of cloche mutants compared with sibling wild-type embryos (insets, dotted lines).