Fig. 8

Fig. 8 Overexpression of bapx1 partially rescues lower jaw elements in wnt9a, frzb and fzd7a morphants. Live embryo morphology (A–C) and Alcian blue cartilage stain (D–F) at 10× magnification, lower jaw flatmount at 20× magnification (G–I). Anterior is to the left in all images. Overexpression of bapx1 mRNA in wnt9a, frzb and fzd7a morphants partially rescued distal palatoquadrate and Meckel′s cartilage elements (arrowhead, D–F, G–I). Graphs summarize the representative phenotypes, where the total number of embryos scored for wildtype (red bar) and morphant phenotypes (blue bar) are shown with 5% error bars (L–N). Overexpression of wnt9a, frzb and fzd7a in HEK293T cells increased Bapx1 protein production as shown by Western blot (J) where actin serves as an internal control and Flag represents mammalian expression vector tag. Bapx1 expression was increased in a statistically significant manner, P<0.05 (K). Abbreviations: M (Meckel′s cartilage), pq (palatoquadrate)