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Fig. S15
Characterization of the novel transgenic RA sensorline. (A) Schematic of the RAR domains and the Gal4 DNA binding domain (GDBD)/RARαb ligand binding domain (RLBD) fusion protein. Grey indicates the GDBD. Yellow indicates the RLBD. D is a linker domain and F is a domain with unknown function (as in Figure 1). (B, C) Schematics representing the GDBD-RLBD fusion acting on the Gal4-UAS:EGFP transgene. The GDBD-RLBD is expressed under the β-actin promoter. (B) In the absence of RA, egfp is not expressed. (C) In the presence of RA (red triangles), the GDBD-RLBD is able to promoted egfp (UAS responsive gene) transcription. (D–G) Tg(β-actin:GDBD-RLB);Tg(UAS:nfsB-mcherry) embryos are responsive to RA treatment. ISH for mcherry. Equivalent results were found when the Tg(β-actin:GDBD-RLB) line was crossed to Tg(UAS:EGFP) fish (data not shown) as were used for experiments in Figure 5. More detailed characterization of the stable transgenic RA sensor lines is reported in 31. (D, E) Lateral views with dorsal right. (F, G) Dorsal views. In images D–G anterior is up.