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Fig. S8
RARαb2 deficient embryos do not have enlarged hearts. (A, B) Hearts from control sibling and RARαb2 deficient Tg(-5.1myl7:DsRed-NLS) embryos at 48 hpf. Images are frontal views. Red indicates ventricle. Green indicates atrium. (C) Mean CM number from the hearts of control sibling and RARαb2 deficient Tg(-5.1myl7:DsRed-NLS) embryos at 48 hpf. (D) qPCR for CM marker gene expression in control sibling and RARαb2 deficient embryos at 48 hpf. We do find a modest decrease in CM number (C) and myl7 expression (D), which is likely due to a very modest amount of MO-induced toxicity.
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