ZFIN Image: Weger et al., 2013, Fig. 7

Image

Figure Caption

Fig. 7

Luciferase reporter and clock gene expression in the suprachiasmatic nucleus. Schematic (upper left corner) indicates the level of the sections across the anterior hypothalamic region shown in the figure. (A) In situ hybridization against luciferase, bmal1 and per1b at two opposite time points of the diurnal cycle (ZT3 and ZT15). Bmal1 and luciferase expression levels are higher at ZT15 than at ZT3, while per1b is higher at ZT3. (B) In situ hybridization of different clock genes at ZT3 (cry1a, per2, per3) and ZT9 (clock3). (C) Schematic indication of anatomical subdomains at the section level shown in (A) and (B) (modified from Wullimann et al. 1996). Abbreviations: Hav, ventral habenular nucleus; VM, ventromedial thalamic nucleus; PPp, parvocellular preoptic nucleus, posterior part; OT, optic tract; SCN, suprachiasmatic nucleus (indicated in all panels by dotted outline). Scale bar, 140 μm.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 380(2), Weger, M., Weger, B.D., Diotel, N., Rastegar, S., Hirota, T., Kay, S.A., Strähle, U., and Dickmeis, T., Real-time in vivo monitoring of circadian E-box enhancer activity: A robust and sensitive zebrafish reporter line for developmental, chemical and neural biology of the circadian clock, 259-73, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.