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Fig. 1

Expression pattern of Notch signalling pathway genes during pectoral fin development.

Whole-mount in situ hybridization of 48 hpf embryos shows strong expression of jagged2 (n = 20) (A), notch1a (n = 22) (B), notch2 (n = 22) (C), notch3 (n = 20) (D), her6 (n = 25) (E), her7 (n = 23) (F), her13.2 (n = 23) (G), mib1 (n = 22) (H) and mib2 (n = 25) (I) in the pectoral fins. At this time point it is possible to detect expression of jagged2 (A′, A′′) and notch3 (D′, D′′) lining the base of the apical ectodermal fold and in the myogenic mesenchyme, notch1a (B′, B′′), her6 (L′, L′′), her13.2 (N′, N′′), mib1 (H′, H′′) and mib2 (I′, I′′) in the myogenic mesenchyme and notch2 (J′, J′′) and her7 (M′, M′′) in the entire fin. (A′–I′) Detached pectoral fins with distal to the top. (A′′-I′′) Transversal sections at the level of pectoral fins. AEF, apical ectodermal fold; dmm, dorsal myogenic mesenchyme; vmm, ventral myogenic mesenchyme; cc, chondrogenic condensation.

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