Fig. 4

Fig. 4

The TNF-RIP1-RIP3 Axis Mediates Necrosis of Infected Macrophages through Mitochondrial ROS Production (A) FPC in WT and LTA4H-high larvae in presence or absence of 10 μM Necrox-5. **p < 0.01 (one-way ANOVA with Tukey’s posttest). Representative of two independent experiments. (B) Percentage of animals in (A) with cording among WT and LTA4H-high larvae in presence or absence of 10 μM Necrox-5. *p < 0.05; ***p < 0.001 (Fisher’s exact test). (C) FPC in WT and LTA4H-high larvae injected with TNF or vehicle in presence or absence of 10 μM Necrox-5. *p < 0.05 (one-way ANOVA with Tukey’s posttest). (D) Percentage of animals in (C) with cording among WT and LTA4H-high larvae injected with TNF or vehicle in presence or absence of 10 μM Necrox-5. *p < 0.05 (Fisher’s exact test). (E) FPC in WT and LTA4H-high larvae in presence or absence of 10 μM Necrosulfonamide (NSA). **p < 0.01; ***p < 0.001 (one-way ANOVA with Tukey’s posttest). Representative of three independent experiments. (F) Mean (±SEM) number of bacteria per infected macrophage in WT and PGAM5 morphant siblings on WT or LTA4H-high background. ***p < 0.001 (one way ANOVA with Tukey’s posttest). Representative of two independent experiments. (G) FPC in WT and PGAM5 morphant siblings on WT or LTA4H-high background. ***p < 0.001 (one-way ANOVA with Tukey’s posttest). Representative of two independent experiments. (H) Percentage of animals in (G) with cording among WT and PGAM5 morphants on WT or LTA4H-high background. **p < 0.01 (Fisher’s exact test). (I–K) Confocal and bright field images of different infected macrophages in 1 dpi larvae 3 (I), 5 (J), or 6 (K) hr post-TNF injection. Scale bars, 1 μm. (Also see Figure S6).