Fig. 1

Fig. 1 Zic2 is required for craniofacial development. Alcian blue-stained cartilage in zic2 morphants at 5 dpf. (A,D) Control morphants have wild-type pharyngeal and neurocranial cartilages (53/55, 6 exp.). (B) Zic2a morphants have variable craniofacial defects, including hypoplastic pharyngeal cartilages and an incorrectly angled hyoid arch (16/54, 5 exp.; outline in 1B′). (E) Severely affected zic2a morphants have more dramatic hypoplasia (10/54, 5 exp.) or ablation of anterior cartilages (11/54, not shown). (C,F) Zic2b morphants are either wild-type (45/70, 6 exp.) or develop shortened mandibular and hyoid arches (25/70, 6 exp.). (G) Penetrance of craniofacial defects in control and morphant embryos. (A–C) are dorsal views with anterior to the left. (D–F) are lateral views. Abbreviations: cb—ceratobranchials, h—hyoid, m—mandibular, nc—neurocranium.