ZFIN Image: Helker et al., 2013, Fig. 7

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Figure Caption

Fig. 7 Vegfc positively regulates EC proliferation in the CCVs. (A-D) Confocal projections of 32 hpf Tg(fli1al:EGFP)^y1 wild-type (A) and homozygous vegfc^hu6410 mutant Tg(fli1al:EGFP)^y1 (B) embryos, and of Tg(kdrl:EGFP)^s843 embryos injected with H2B-cherry mRNA (control, C) or vegfc mRNA (D). (E) Quantification of EC numbers and proliferation rates in the CCVs. EC proliferation is decreased in vegfc^hu6410 or sFLT4 mRNA-injected embryos (B,E) and increased upon vegfc overexpression (D,E). Embryos were pulsed with BrdU from 24-32 hpf. (F,G) Expression of flt4 in the CCV (F, arrowheads) or vegfc in the area of CCV formation (G, bracket) detected by in situ hybridization at 29 hpf (F) or 32 hpf (G) in dorsal (F) or lateral (G) view. ^***P<0.001; error bars indicate s.e.m. Ctr, control.

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Acknowledgments

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