IMAGE

Fig. 3

ID

ZDB-IMAGE-130719-13

Genes

Antibodies

Ab2-ctnnb1

Source

Figures for Neal et al., 2013

Image

Figure Caption

Fig. 3 CagA activates canonical Wnt signaling in the intestinal epithelium. (A–C) Quantitative RT-PCR data showing relative expression levels of the Wnt target genes mycA (A), cyclinD1 (B) and axin2 (C). Expression levels were assayed in dissected adult intestines and normalized to SDHA and β-actin; bars indicate mean ± s.d. of biological triplicates. (D–G) Immunofluorescence micrographs showing proliferating cells (EdU, green, 10-hour label) and cells with nuclear and cytoplasmic accumulation of β-catenin (red staining and white arrowheads) in intestinal cross-sections of wild-type (D), b-cagA (E), axin1^tm213 (F) and b-cagA; axin1^tm213 (G) animals at 6 dpf. (H) Quantification of proliferating (EdU+) cells. (I) Quantification of cells with nuclear and cytoplasmic accumulation of β-catenin.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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