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Fig. 7 Modulation of sfrp5 expression causes defects in gastrointestinal development.
A–P) Dorsal views of 3 dpf old embryos stained with probes against fabp10a (A–D), anxa2b (E–H), try (I–L), and ins (M–O). Embryos were injected with 0.5 pmol mismatch morpholino (A, E, I, M), 0.5 pmol morpholino against sfrp5 (B, F, J, N), 100 pg eGFP mRNA (C, G, K, O) or 50 pg sfrp5 mRNA (D, H, L, P). Q) Chart summarizing in situ results (A–L), showing percentages of normal or small GI organs. R) Chart summarizing in situ results (M–P), showing the percentage of larvae in which ins+ cells failed to coalesce. The total number of analyzed embryos per treatment is shown below each column.