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Fig. 7 Effect of HDAC inhibition on sympathetic neuron differentiation is reversible.
A, B wild-type embryos treated with DMSO (A) and TSA (B) continuously from 28–52 hpf and then fixed at 52 hpf and stained for th expression, black arrowheads indicate sympathetic neurons. C–E wild-type embryos treated under three different conditions, C with DMSO between 28–72 hpf, D with TSA between 28–72 hpf, and E with TSA between 28–52 hpf after which the TSA is washed out and then the embryos are treated in DMSO between 52–72 hpf. All embryos were fixed at 72 hpf and then stained for th expression. Black arrowheads indicate th-xpressing sympathetic neurons, or their absence.