ZFIN Image: Lin et al., 2013, Fig. 5

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Figure Caption

Fig. 5

Rescue of prmt8 or prmt1 morphants with prmt8 or prmt1 cRNA.

(A) Frequencies of normal, dead or defective embryos not injected (WT), injected with 2 ng of prmt8 MO or prmt8 MO with full-length prmt8 (FL) cRNA, catalytic inactive mutant prmt8 (CI MT) cRNA, full-length prmt1 cRNA, prmt8 N-terminus with full-length prmt1 (prmt8+1) cRNA, N-terminus-deleted prmt8 (ND) cRNA, or G2A mutated prmt8 cRNA. The embryos were examined and classified as described in Figure 3B. (B) Frequencies of different phenotypes of embryos not injected (WT), injected with 2 ng of prmt1 MO, or prmt1 MO with full-length (FL) prmt1 cRNA, full-length prmt8 cRNA, N-terminal deleted (ND) prmt8 or prmt8 N-terminus with full-length prmt1 (prmt8+1) cRNA Injected embryos were stained with ntl at 10 hpf and the morphants were grouped according to the degree of shortening and widening of the notochord.

Acknowledgments

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