ZFIN Image: Lu et al., 2013, Fig. 2

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Figure Caption

Fig. 2 EpCAM Acts Downstream of Wnt2bb in Liver Development through Its Extracellular Domain(A–E) At 102 hpf, liver phenotypes in hi2151 mutants (B, 37/50) can not be rescued by wnt2bb mRNA (C, 33/46), whereas reduced liver size in wnt2bb morphants (D, 66/90) is rescued by epcam mRNA (E, 73/99) as shown under the Tg(lfabp:GFP) background.(F–O) Expressions of hhex (F–J, arrowheads) at 28 hpf and cp (K–O) at 52 hpf in the hepatic endoderm.(P–R) Antibody stainings illustrate the membrane enrichment of EpCAM on hepatocytes at 52 hpf (P), which is extensively colocalized with the membrane GFP signal under the Tg(rasGFP) background (Q and R).(S–U) Defective liver development in wnt2bb morphants at 102 hpf (D, 66/90) is rescued by either epcam ECD-TM (S, 51/66) or epcam ECD (T, 51/75) mRNA, but not rescued by epcam ICD mRNA (U, 72/78).See also Figures S1, S3, and Table S1.

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Acknowledgments

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Reprinted from Developmental Cell, 24(5), Lu, H., Ma, J., Yang, Y., Shi, W., and Luo, L., EpCAM Is an Endoderm-Specific Wnt Derepressor that Licenses Hepatic Development, 543-553, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell