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Fig. 1 Liver Development Is Impaired in hi2151/epcam Zebrafish Mutant (A) Western blotting on the embryonic lysates at 24 hpf using antibodies against the ICD of zebrafish EpCAM. α-Tubulin serves as a loading control.(B) Except smaller otoliths, there is no obviously visible phenotype in hi2151 mutant embryos at 52 hpf.(C and D) Liver bud in hi2151 mutants (D, 41/60) is smaller than that in the wild-type (C, 55/56) at 52 hpf as observed under the Tg(gutGFP) transgenic background. L, liver; P, pancreas.(E–G) In contrast to the wild-type (E, 50/50), liver size is reduced in hi2151 mutants at 102 hpf (F, 52/72), whereas injection of epcam mRNA leads to enlarged liver (G, 45/59) as shown under the Tg(lfabp:GFP) transgenic background.(H–O) Expressions of hepatoblast specification markers hhex (H, 32/35; and I, 29/40; arrowheads) and prox1 (J, 36/37) (K, 28/37) at 28 hpf as well as differentiation markers cp (L, 39/41) (M, 27/39) and sePb (N, 31/34) (O, 30/38) at 52 hpf.See also Figures S1, S2, S6, and Table S1.
Reprinted from Developmental Cell, 24(5), Lu, H., Ma, J., Yang, Y., Shi, W., and Luo, L., EpCAM Is an Endoderm-Specific Wnt Derepressor that Licenses Hepatic Development, 543-553, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell