ZFIN Image: Lu et al., 2013, Fig. S5

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Figure Caption

Fig. S5 kremen1 Is Present in the Endoderm at 24 hpf, and Its Roles in Early Endoderm and Liver Development. related to Figure 4
(A) kremen1 (krm1) is ubiquitously expressed at 24 hpf.
(B and C) Endodermal cells are collected by sorting out GFP positive cells from Tg(sox17:GFP) transgenic embryos.
(D) Presence of kremen1 in the endoderm is confirmed by RT-PCR using sorted endodermal cells. Transcriptions of foxa3 and otx2 are applied as positive and negative controls, respectively.
(E and F) In contrast to the wild-type (E), injection of kremen1 mRNA results in slightly increased number of endodermal cells and mildly expanded endoderm at the 14-somite stage (F) as observed under the Tg(sox17:GFP) background.
(G and H) Injection of kremen1MO leads to reduced number of endodermal cells (G), which is rescued by the co-injection of kremen1 mRNA (H).
(I and J) Expressions of Kremen1-GFP at 26 hpf caused by heat shock of Tg(hsp70l: kremen1-GFP) transgenic embryos at 18 hpf.
(K and L) In contrast to the Kremen1-GFP negative embryos (K), liver development is aberrant in the Kremen1-GFP positive embryos at 102 hpf (L).

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Acknowledgments

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Reprinted from Developmental Cell, 24(5), Lu, H., Ma, J., Yang, Y., Shi, W., and Luo, L., EpCAM Is an Endoderm-Specific Wnt Derepressor that Licenses Hepatic Development, 543-553, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell