ZFIN Image: Ding et al., 2013, Fig. 3

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Figure Caption

Fig. 3 prdm3 and prdm16knockdown causes a reduction in the NCC expression domains of dlx2a at 24 hpf and barx1 at 48 hpf. A-C: Whole mount embryo ISH shows that pharyngeal arch marker dlx2a is expressed in the post-migratory neural crest region in control (A), prdm3 e3i3 (B), and prdm16 ATG (C) morphants at 24 hpf. dlx2a expression in prdm3 and prdm16 morphants shows a significant reduction in the anterior expression domain, and a mild reduction anteriorly relative to controls. D-F: barx1 expression in cranial NCC condensations is significantly reduced in the neurocranium (n) and arch 1 (1), dorsal and ventral domains within arch 2 (2d and 2v), and a domain representing arches 6 and 7 (6/7) in prdm3 (E) and prdm16 (F) morphants at 48 hpf as compared to controls (D). Arrows indicate notably reduced expression domains in prdm3 and prdm16 morphants. Anterior is to the left.

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Acknowledgments

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