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Fig. 4 Knock down of contactin-2 expression after spinal cord injury retards locomotor recovery after spinal cord injury.
(A) Contactin-2 protein expression is reduced at 10 days after SCI by application of contactin-2 antisense morpholino (MO) as detected by Western blot analysis. (B) Intensity of the bands as quantified by ImageJ software, and fold change compared with control MO treated group shows a decrease in contactin-2 protein level at 10 days after application of contactin-2 MO (*p<0.05, Student’s t-test; n = 5 fish/group). Values represent means+SEM. (C) Total distance moved in 5 min of undisturbed zebrafish treated with contactin-2 MO or control MO (n = 8 fish/group) after SCI. At 3 and 5 weeks after SCI, the total distance moved was reduced by treatment with contactin-2 MO when compared to fish treated with control MO (*p<0.05, one-way ANOVA with post-hoc Student-Newman-Keuls test; n = 8 fish/group). Values represent means+SEM.