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Fig. 3 T-cell development is impaired in zebrafish cdca7 and mcm2 morphants. (A) The protein expression of Cdca7 and Mcm2 in the zebrafish cdca7 and mcm2 morphants detected by using Western blot. (B) The expression of T-cell markers (rag1, il7r, and ikaros) in zebrafish cdca7 and mcm2 morphants by qRT-PCR (mean ± SD, Student’s t test, **P < 0.01, n = 3). (C) The expression of rag1, il7r, ikaros, and foxn1 in zebrafish cdca7 and mcm2 morphants by WISH. Anterior to the left and dorsal to the up; circles mark the thymus.
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