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Fig. 4 Fgf Signaling Is Required for Injury-Induced Gata3 Expression(A) Heat-shock scheme.(B) Gata3 IHC on 3 dpl heat-shocked sham control fish telencephalon.(C) Gata3 IHC on 3 dpl heat-shocked Tg(hsp:dnfgfr1-gfp) fish telencephalon. Inset: double IHC for GFP and Gata3, showing uniform dnFgfr1-GFP.(D) High magnification from dorsomedial region of (B) with DAPI.(E) High magnification from dorsomedial region of (C) with DAPI.(F) gata3 ISH in heat-shocked control telencephalon at 3 dpl.(G) gata3 ISH in heat-shocked Tg(hsp:dnfgfr1-gfp) telencephalon at 3 dpl. Scale bars 20 μm. n = 4 fish for (B), 6 for (C), 6 for (D and E), and 5 for (F and G). Data are represented as mean ± SEM.(H) Heat-shock and amputation scheme for Tg(hsp70l:dnfgfr1) fish. (H′) gata3 ISH on 2 dpa nontransgenic siblings. (H′′) gata3 ISH on 2 dpa Tg(hsp70:dnfgfr1) fish.(I) Transient and brief heat-shock and amputation scheme for Tg(hsp70l:dnfgfr1) fish. (I2) Gata3 and GFP immunostaining on nontransgenic siblings shown for single fin ray. Dashed lines, amputation margin. (I′1–I′3) Individual fluorescence channel for Gata3, DAPI, and merged image. (I′′) Gata3 and GFP immunostaining on Tg(hsp:dnfgfr1-egfp) animals shown for single fin ray. Dashed lines, amputation margin.(J) spry4 expression at 24 hr postfertilization (hpf) embryo.(K) Blocking Fgf signaling abrogates spry4 expression.(L) gata3 expression at 24 hpf.(M) Blocking Fgf signaling does not alter the expression of gata3. (J′–M′) Dorsal view of animals in (J, K, L, and M), respectively. n = 5 for (H–H′′), 3 for (I–I′′), and more than 50 embryos for (J–M2). Scale bars 200 μm.See also Figure S4.
Reprinted from Developmental Cell, 23(6), Kizil, C., Kyritsis, N., Dudczig, S., Kroehne, V., Freudenreich, D., Kaslin, J., and Brand, M., Regenerative Neurogenesis from Neural Progenitor Cells Requires Injury-Induced Expression of Gata3, 1230-1237, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell