Fig. 8

Fig. 8 Effects of co-misexpressing pairs of competence factors in Bmp-blocked embryos. A–E, Expression of competence factors tfap2a (A) and tfap2c (B) at 10 hpf, PPE marker dlx3b at 11 hpf (C), NC marker foxd3 at 11 hpf (D), and two-color in situ hybridization to visualize patterns of dlx3b and foxd3 (E) in controls, embryos treated with 200 μM DM from 4 hpf, and hs:foxi1/+; hs:gata3/+ transgenic embryos treated with 200 μM DM from 4 hpf and heat shocked at 6 hpf. All markers show rescue in DM-treated embryos following transgene activation. Two-color staining in (E) shows a dorsolateral view of the hindbrain area in a control embryo, a lateral view of a whole DM-treated embryo (dorsal to the right), and a close-up of the ventral midline of a rescued DM-treated transgenic embryo in which dlx3b and foxd3 are expressed in intermingled, largely non-overlapping clumps of cells. Scale bars, 100 μm. F and G, Expression of otic/epibranchial marker pax8 at 11 hpf (F) and trigeminal/anterior lateral line marker neurod at 14 hpf (G) in control embryos, embryos treated with 200 μM DM from 4 hpf, or in embryos carrying the indicated combinations of transgenes treated with 200 μM DM from 4 hpf and heat shocked at 6 hpf. Arrows point to weak, scattered expression of pax8 or neuroD in various backgrounds.