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Fig. 3 Lateral positioning of longitudinal axons derived from otpb- and hypocretin-positive neurons is altered in ast homozygous mutants. Dorsal views of hindbrain confocal z-projections of otpb:gfp;hcrt:tdtomato double transgenic 72 hpf embryos. (A-F) In ast/ast embryos longitudinal projections of otpb:gfp (D,F; arrows in D) and hcrt:tdtomato (E,F; arrowheads in E) axons are shifted towards the midline compared with ast/+ siblings. (G) Quantification of the distance between otpb:gfp and hcrt:tdtomato HTS axons at the anterior-posterior level of Mauthner neurons, and of the distance between Mauthner neurons. ***P<0.0001; n.s., not significant. Scale bar in A for A-F: 50 μm.