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Fig. 2 prdm14 is trapped in the slg mutant. (A) Boundary sequences of the inserted transposon and prdm14 gene. (B,C) Zebrafish Prdm14 protein contains a SET domain and a six zinc-finger domain, which are highly conserved. z, zebrafish; m, mouse; h, human. (D) PCR genotyping confirms that the prdm14 gene is disrupted in slg mutants (right). Heterozygotes and homozygotes show different GFP intensities (left). (E-M) prdm14 RNA expression patterns detected by in situ hybridization. prdm14 is expressed in neural plate at 30% epiboly (E) and in the anterior neural plate at 75% epiboly and bud stages (F,G). At the 1-somite stage, prdm14 RNA is restricted to dorsal and ventral neuron precursors and precordal plate (H,K) and at the 10-somite stage prdm14 is detected in neuron precursors of the spinal cord (I,L). At the 22-somite stage prdm14 is further restricted to the ventral midbrain, olfactory sensory neurons, statoacoustic neurons, trigeminal neurons and spinal cord (J,M). (N-O′′) prdm14 RNA is co-expressed with ngn1 at the 3-somite stage as detected by double in situ hybridization. (P-P′′) prdm14 is co-expressed with islet2 in RB and CaP at the 26-somite stage. Animal view, dorsal to the top in E-G; anterior view, dorsal to the top in H-J,N-N′′; dorsal view, anterior to the left in K,L; lateral view, anterior to the left in M,P-P′′; dorsal view, anterior to the bottom in O-O′′. N, neural plate; AN, anterior neural plate; V and D, ventral and dorsal neuron precursors; PP, precordal plate; SC, spinal cord; M, ventral midbrain; O, olfactory sensory neurons; S, statoacoustic ganglion neurons; T, trigeminal neurons. Scale bars: 500 μm in D; 200 μm in E-P′; 100 μm in N′′-P′′.