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Fig. 3 Effect of Cdc14A1 loss of function in LR asymmetry establishment. ISH and expression pattern quantification in non-injected, cdc14a1 and cdc14b MO- injected embryos. A-D: nkx2.5 expression at 31 hpf. E–H: foxa3 expression at 50 hpf. I-L: spaw expression at 19 hpf. Scale bar = 100 μm. M: Rescue of LR asymmetry defect in Cdc14A1-depleted embryos. Heart tube shifting was determined at 31 hpf. Numbers of embryos analyzed are indicated. Data are represented as mean ± SEM. Statistics were done using Student′s t-Test (D,H,L) and are only presented for the left-sided heart tube class in M.
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