ZFIN Image: Liu et al., 2012, Fig. S2

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Figure Caption

Fig. S2 Git1 mRNA expression pattern and knockdown vascular phenotype

(AC) In situ hybridization with a RNA probe against Git1 reveals ubiquitous staining at 1 day post fertilization (dpf) and 2 dpf. At 3 dpf, staining is mostly restricted to the head, but is still broadly distributed. (DF) Confocal microscopy of wild type head (D) and trunk (F) or Git1^i1e2 morphant head (E) or trunk (G) at 3 dpf shows a largely normal vascular pattern in Tg(kdrla:GFP)^la116 embryos. As compared to wild type embryos, the head vasculature in the region of the central arteries (CtAs) is underdeveloped (arrow). (H) Amplification of Git1 with primers surrounding the deleted exon shows that Git1 mRNA is absent in Git1^i1e2 morphants, while the control gene EF1α amplifies normally. Scale bar in all panels represents 100 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 363(1), Liu, J., Zeng, L., Kennedy, R.M., Gruenig, N.M., and Childs, S.J., betaPix plays a dual role in cerebral vascular stability and angiogenesis, and interacts with integrin alphavbeta8, 95-105, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.