Fig. S4
Nuclear Envelope Assembly during Midgastrulation, Related to Figure 6(A) One set of separating chromosomes (single Z-slice) at 1.75 hpf (comparable time point to 5B) stained with Bmb (green) and mab414 (red).(B–E) Anaphase-interphase transition at 8.0 hpf. In (B) and (C) Bmb (left, green) and mab414 (middle, red) are not detected on the separating chromosomes (arrows). In (D) Bmb and mab414 are now detected on the single chromatin mass and the fully formed nucleus (E). Scale bar = 5 μm. Panels only show a single Z-slice to illustrate individual chromosomes versus a single chromatin mass. Each time point n e 3.
Reprinted from Cell, 150(3), Abrams, E.W., Zhang, H., Marlow, F.L., Kapp, L., Lu, S., and Mullins, M.C., Dynamic Assembly of Brambleberry Mediates Nuclear Envelope Fusion during Early Development, 521-532, Copyright (2012) with permission from Elsevier. Full text @ Cell