ZFIN Image: Abrams et al., 2012, Fig. 3

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Figure Caption

Fig. 3

bmb Encodes a Conserved Predicted Transmembrane Protein(A) Recombinants over total meiotic events are indicated parenthetically in red below the marker name. Candidate genes eliminated based on their WT cDNA sequence are indicated with a red X. A histone gene cluster is indicated as red boxes. Two predicted transposable elements are indicated by black boxes.(B) ORF1.8 encodes a 612 residue protein with a predicted N-terminal signal peptide sequence (SP) followed by a BHD, a coiled-coil (CC), and two C-terminal transmembrane domains (TM) initiating at amino acid residues 363 and 415 (TMpred program) (Hofmann and Stoffel, 1993). Yeast Kar5p is 27% similar (excluding the C-terminal region) to the D. rerio homolog. The red bar corresponds to the recombinant protein used to generate Bmb antibodies.(C) Rescued bmb embryos at 50% epiboly (5.5 hpf) and 24 hpf.See also Figure S1.

Acknowledgments

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Reprinted from Cell, 150(3), Abrams, E.W., Zhang, H., Marlow, F.L., Kapp, L., Lu, S., and Mullins, M.C., Dynamic Assembly of Brambleberry Mediates Nuclear Envelope Fusion during Early Development, 521-532, Copyright (2012) with permission from Elsevier. Full text @ Cell