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Fig. S1 The sq198 mutation affects organ growth but does not affect the specification and early development of major digestive organs. (A) sq198 was identified in our screening as a liver defective mutant using prox1 as a liver marker. (B) sq198 conferred smaller liver, unexpanded swimbladder and much thinner intestine with an unabsorbed yolk as seen under a dissection microscope at 5 dpf. The liver is indicated by a red arrow. The intestinal tract is traced with green line and is indicated with a green arrow. WT: wildtype; mu: mutant; in: intestine; lv: liver. (C-I) WISH using early liver markers prox1 (C,F) and hhex (D), pan-endoderm markers foxA1 (E), gata6 (G) and shh (H), and early pancreatic marker pdx1 (I) on embryos at 1 dpf or 2 dpf as indicated. No observable phenotypic differences between the wildtype (WT) and mutant (mu) for these markers are found during the stages examined. Red arrow: liver; blue arrow: pancreas; green arrow: intestine.
Reprinted from Developmental Biology, 367(2), Niu, X., Gao, C., Jan Lo, L., Luo, Y., Meng, C., Hong, J., Hong, W., and Peng, J., Sec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafish, 197-207, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.