ZFIN Image: Jia et al., 2012, Fig. 7

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Fig. 7

Downregulation of Hdac3 Activity Counteracts ppp4cb-MO1-Induced Embryonic Dorsalization (A and B) bmp2b mRNA-induced gsc reduction (dorsal views) and gata2 expansion (animal-pole views) were rescued by coinjection of HDAC3 mRNA. Statistical data are shown in (B). (C and D) ppp4cb-MO1 (bMO1)-induced gsc expansion (dorsal views) or eve1 reduction (animal-pole views) was inhibited by coinjection of VPA. Statistical data are shown in (D). (E and F) ppp4cb-MO1 (bMO1)-induced gsc expansion (dorsal views) or eve1 reduction (animal-pole views) was blocked by coinjection of 10 ng hdac3-MO, but not by coinjection of 10 ng hdac1-MO. Statistical data are shown in (F). (G) Schematic diagram summarizes the working model of Ppp4c in regulating BMP signaling. In response to BMP, Ppp4c is recruited to the Smad1/Smad5-occupied promoter and dephosphorylates/inactivates Hdac3. The HAT complex most probably associates with the heteromeric Smads complex, catalyzes the histone tail acetylation, and ultimately favors the transcriptional activation.

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Acknowledgments

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Reprinted from Developmental Cell, 22(5), Jia, S., Dai, F., Wu, D., Lin, X., Xing, C., Xue, Y., Wang, Y., Xiao, M., Wu, W., Feng, X.H., and Meng, A., Protein Phosphatase 4 Cooperates with Smads to Promote BMP Signaling in Dorsoventral Patterning of Zebrafish Embryos, 1065-1078, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell