ZFIN Image: Tu et al., 2012, Fig. S6

Image

Figure Caption

Fig. S6 Knockdown of Arl6ip1 induces KV cilia defects. (A-D) pitx2 normal expression in left lateral plate mesoderm (LPM)(arrow) at 20-somite stage was disrupted in embryos injected with arl6ip1-MO1/p53-MO. (E-H) At 20-somite stage, spaw is expressed in the left LPM (arrow), while knockdown of Arl6ip1/P53 resulted in left (E), absent (F), bilateral (G) or rightward (H) spaw expression in the LPM. (I, J) Confocal microscopic images of anti-acetylated tubulin staining of KV cilia (red fluorescent) at 8-somite stage. In the wild-type embryos, cilia can be observed in a spherical pattern in the region of KV (I). Cilia number, length and KV area reduced in arl6ip1-MO1/p53-MO morphants at 8-somite stage (J). (K) Summary of pitx2 and spaw asymmetrical gene expression patterns.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One