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Fig. S1
Muscle and ovary tissue do not exhibit CD31 staining. Immunohistochemistry on transversal sections from a ptena+/-ptenb-/- mutant fish with a diagnosed hemangiosarcoma, using CD31 specific antibody. Different areas from the CD31-stained sections in Fig. 4 are depicted here, which contain (a) muscle and (b) ovary. Both do not show CD31 staining, as expected for these non-endothelial tissues.. Sections of representative tissues are depicted. Scale bar is 20 μm, as indicated.
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