ZFIN Image: Muto et al., 2011, Fig. 8

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Figure Caption

Fig. 8 Distinct effects of Nipbl and cohesin on gene expression.

(A) Expression of sox32 (upper) and sox17 (lower) in uninjected (left column), nipbla/b-morphants (middle column) and smc3-morphants (right column) was examined by in situ hybridization at 8.3 hpf (80% epiboly). Views are dorsal with anterior at top. (B) Gene expression changes in nipbla/b- and cohesin-morphants. Gene expression was examined in embryos injected with nipbla/b-MO1, smc3-MO, and rad21-MO by Q-PCR at 6.5 hpf or 26 hpf (as indicated). Expression levels are presented relative to those in uninjected embryos (100%; n = 3, * p<0.01, ** p<0.05). (C) Levels of Smc3, Nipbla, and Nibplb protein were examined by Western blotting at 9 hpf. α-Tubulin was used as a loading control. Amounts of MO injected in each embryo were: nipbla/b-MO1, 0.75 ng each; smc3-MO, 4 ng; rad21-MO, 5 ng. (D) Gross morphologies of cohesin-morphants at 25 hpf. Views are lateral with anterior to the left.

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Acknowledgments

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