ZFIN Image: Muto et al., 2011, Fig. 7

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Figure Caption

Fig. 7 L/R patterning defects in nipbla/b-morphants.

Left-sided expression of spaw (n = 38) and lefty2 (n = 60) in lateral plate mesoderm (LPM) is disrupted in nipbla/b-morphants. (A–D) At 18 hpf (18-somite), spaw expression (red arrows) was variably unchanged (B; 47.4% of embryos), absent (C; 39.4%), or bilateral (D; 13.2%). The midline is marked by a dotted line. Insets show bilateral expression of spaw in the tail-bud. (E–I) lefty2 expression at 21.5 hpf (25-somite) was either unchanged (F; 34.9%), reduced (G; 33.3%), absent (H; 25.4%) or right-sided (I; 6.3%). Views are dorsal, anterior to the top. (J–M) Morphology of Kupffer′s vesicle (J, K; arrowheads; bright-field microscopy) and charon expression (L, M; ISH; posterior views of tail regions) are similar in control and nipbla/b-morphants at 16 hpf (14-somite). (N–P) Expression of dnah9 in dorsal forerunner cells (arrows) at 8.3 hpf (80% epiboly) is reduced in nipbla/b-morphants (O, P) relative to controls. Views are dorsal, anterior to the top. Scale bars: 50 μm.

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Acknowledgments

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