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Fig. 3 Supernumerary Mauthner axons are ensheathed by myelinating glia. (A-C) Ventral views of the hindbrains of wild-type (A), notch1a morphant (B) and hoxb1 mRNA-injected (C) larvae at 5 dpf labelled with the 3A10 antibody, which recognises neurofilament-associated proteins. Mauthner axons are indicated by arrowheads. Scale bar: 20 μm. (A′-C′) Analysis of Tg(sox10:mRFP) (A′-C′) and Tg(mbp:EGFP-CAAX) (A′-C′) shows that wild-type (A′,A′), notch1a morphant (B′,B′) and hoxb1-injected (C′,C′) Mauthner axons are all ensheathed and myelinated. Arrowheads indicate Mauthner axons. Scale bars: 20 μm. (D) RT-PCR analyses show that levels of notch1a mRNA are reduced in morpholino (MO)-injected animals relative to controls at 10 hpf but not at 72 hpf.