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Fig. 7
Lefty1 is required for Bmp induced repression of spaw.
In situ hybridisation of spaw (in LPM) and ntl (in midline) at 18-somites. Wild-type (A,C) or Tg(hsp70l:bmp2b) (B,D) embryos were heat-shocked at 10 hpf to induce bmp2b expression (B,D). Ectopic expression of bmp2b resulted in the loss of spaw expression in the LPM (B). A subset of embryos were injected with a lefty1 MO (C,D), which resulted in bilateral spaw expression even in the presence of ectopic bmp2b (D). Embryos are shown as dorsal views with anterior to the top and left to the left.
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