ZFIN Image: Smith et al., 2011, Fig. 4

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Figure Caption

Fig. 4

Rescue of Bmp-related cardiac laterality defects by Nodal beads.

In situ hybridisation for myl7 to highlight the position of the linear heart tube at 30 hpf. Tg(hsp70l:nog3) embryos with no heat-shock (A,D) or heat-shocked at 16 hpf (B,E). Beads (blue) preincubated with recombinant Nodal protein placed in the right ALPM of non-heat-shocked (D) or heat-shocked (E) Tg(hsp70l:nog3) embryos at 17–18 hpf. Control siblings (G,J) or MZbmpr1aa mutant embryos (H,K). Beads (blue) preincubated with Nodal protein placed in the right ALPM of siblings (J) or MZbmpr1aa mutant embryos (K). Position of the inflow pole of the linear heart tube was determined for embryos without a Nodal bead (C) and for embryos in which a Nodal bead was placed on the right side (F). Embryos are shown as dorsal views with anterior to the top and left to the left.

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Acknowledgments

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