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Fig. 5
Overexpression of human NRG1 type III in all neurons leads to expression of Schwann cell markers in the spinal cord. (A-J) Lateral views of human NRG1 (hNRG1) type III-overexpressing transgenic zebrafish embryos (B,D,F,H,J) and control siblings (A,C,E,G,I) stained by in situ hybridization for the indicated genes. Anterior is to the left and dorsal is up. Embryos were genotyped before imaging. Transgenic embryos have expanded foxd3 (C,D), mbp (E,F) and krox20 (I,J) expression in dorsal hindbrain (brackets) and spinal cord (arrowheads). olig2 is only slightly expanded in hNRG1 type III-overexpressing embryos (G,H). (K-M) Cryosections of embryos showing krox20 expression. krox20-expressing cells are present within the neural tube (black arrowheads) and along nearby nerves (asterisks) of hNRG1 type III-overexpressing embryos (L,M), but not in control embryos (K). The PLLn is marked by open arrowheads.