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Fig. 3 Embryos injected with cdh2 morpholino antisense oligonucleotides (cdh2MO) phenocopy the pac and the glass onion mutants. A–E: Views from 24 hours postfertilization (hpf) embryos with anterior to the left and dorsal up. B illustrates defects in the midbrain and hindbrain (H). The arrows in A and B point to the midbrain–hindbrain boundary. The arrowhead in B points to loose cell aggregates in the fourth ventricle. Compared with the tip of the tail in control embryos (C), cdh2MO embryos had split fin (D) or tail blister (E). F,G: Cross-sections (dorsal is up) of Cdh2 immunostaining of control morpholino oligonucleotide (Control) -injected (F) and cdh2MO-injected embryos (G), both at 50 hpf. dm, dorsal musculature; ed, endoskeletal disc; Lb, limb bud; vm, ventral musculature.