ZFIN Image: Chang et al., 2011, Fig. 7

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Fig. 7

Hypoplastic liver in leg1-MO^ATG morphants is caused by cell cycle arrest during the liver budding stage.

(A, B) Immunostaining using an anti-PH3 antibody on st-MO (A) and ATG-MO (B) morphants at 38hpf. Liver primordium is circled with a white dotted line. (C, D) Immunostaining using antibodies respectively against PH3 (red) and RFP (green) on cross-sections from st-MO (C) and ATG-MO (D) morphants in the Tg(lfabp: DsRed; elaA: EGFP) background at 3dpf. (E) Statistical analysis revealed that the ratio of PH3-positive cells was significantly reduced in the liver primordium of ATG-MO morphants at 38 hpf, but no significant difference in the ratio of PH3-positive cells was observed in the liver of ATG-MO morphants at 3dpf. Data was obtained by counting PH3-positive cells versus total cells in a specific organ (e.g. liver) in sections from at least 3 st-MO embryos and 3 ATG-MO morphants at each stage, respectively (Table S1).

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Acknowledgments

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