ZFIN Image: Bricaud et al., 2011, Fig. S6

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Figure Caption

Fig. S6

Otic neurons (upper panels, in green; pointed to by arrowheads and surrounded by a dotted line in first panel) and anterior macula hair cells (lower panels, in red) in 3 dpf zebrafish larvae in which Gro1 has been knocked-down by morpholinomodified oligonucleotide injection (B, E), or in which the effect of the Gro1 knock-down has been rescued by injection of Gro1 mRNA (C, F). Otic neurons have been detected by anti-HuC immunostaining whereas hair cells of the anterior macula have been detected by anti-HCS-1 immunostaining. Gro1 mRNA has been generated from a Nterminal HA (Hemagglutinin A)-tagged gro1 cDNA, generated by cloning the zebrafish gro1 cDNA in phase into the pCMV-HA vector (Clontech, Mountain View, CA, USA). Adding the HA tag perturbs the integrity of the MO-binding site on gro1 mRNA, preventing binding of the MO. Upper panels are lateral views with a scale bar of 30 μm, lower panels are ventral views with a scale car of 20 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 357(1), Bricaud, O., and Collazo, A., Balancing cell numbers during organogenesis: Six1a differentially affects neurons and sensory hair cells in the inner ear, 191-201, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.