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Fig. 6
Swim bladder formation requires Wnt2, Wnt2bb and Fzd5 activity. foxa1 (A-E) and agr2 (F-I) expression at 48 hpf shows that the swim bladder (outlined by dashed line) is variably reduced in MO-wnt2 (B,G), prt/wnt2bb (C,H) MO-fzd5 (E) and absent in MO-wnt2;prt/wnt2bb embryos (bracket, D,I) compared with controls (A,A2,F,F2). Dorsal views, anterior to top. (J) Proliferation rates in controls, prt/wnt2bb and MO-wnt2 embryos determined by Tg(XlEef1a1:GFP)s854 and BrdU labelling at 42 hpf. Data are mean±s.e.m. P values are shown (**P<0.005); n>13 embryos. L, liver; sb, swim bladder.
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